Partlow is an unincorporated community in Spotsylvania County in the U.S. state of Virginia.

Partlow's primary historical significance is as the location of the plantation where Kunta Kinte was enslaved. Partlow is also home to Wallers Baptist Church, a historically notable church founded in 1769, known for the notorious persecutions against its founding pastor, John "Swearing Jack" Waller, by the Church of England.

Originally, the area was termed "Pea Ridge" and was the site of a slave auction. Part of the original slave block still rests directly on Rt. 738 between the Partlow Post Office and Mt. Olive Rd (Rt. 658). A tavern was established by Capt. John C. Partlow on the same corner as the slave block called "The Partlow Tavern". Capt. Partlow, along with Benjamin Waller, a descendant of John Waller (founder of Waller's Church) were persecuted for their religion and briefly jailed at one point for having services in their homes.